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KMID : 1161520100140040237
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Animal Cells and Systems
2010 Volume.14 No. 4 p.237 ~ p.244
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Transplantation of human adipose-derived stem cells into the urethra ameliorates stress urinary incontinence and blunts the induction of c-Fos immunoreactivities in brain areas related to micturition in female rats
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Kim Sung-Eun
Ko Il-Gyu
Kim Bo-Kyun
Sung Yun-Hee
Shin Mal-Soon
Cho Se-Hyung
Kim Chang-Ju
Kim Khae-Hawn
Lee Kyo-Won
Kim Dong-Hee
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Abstract
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Stress urinary incontinence (SUI) is a common condition that primarily affects women. Here, we investigate the effects of human adipose-derived stem cells (ADSCs) in a rodent model of SUI. Female Sprague-Dawley rats at 7 weeks of age were randomly divided into three groups (n=8 per group): sham-operation, SUI-induction by transabdominal urethrolysis, and SUI-induction followed by transplantation of human ADSCs into the urethra. The abdominal leak point pressure at 8 weeks after the operation was markedly decreased by transabdominal urethrolysis, confirming successful induction of SUI. Interestingly, transplantation of human ADSCs into the urethra significantly blunted the decrease of abdominal leak point pressure in SUI-induced rats. Accordingly, we observed expression of α-smooth muscle actin in a significant proportion of transplanted ADSCs, indicating differentiation of ADSCs into smooth muscle cells in the urethra. Moreover, the SUI-induced elevations of c-Fos immunoreactivities in the pontine micturition center (PMC) and in the ventrolateral periaqueductal gray (vlPAG) were clearly suppressed by transplantation of human ADSCs. These results imply that human ADSCs can be an effective therapeutic modality to ameliorate the symptoms of SUI.
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KEYWORD
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stress urinary incontinence, human adipose-derived stem cells, abdominal leak point pressure, a-smooth muscle actin, c-Fos
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